Gene expression analysis of human hepatocellular carcinoma by using full-length cDNA library
نویسندگان
چکیده
منابع مشابه
Gene Organization in Rice Revealed by Full-Length cDNA Mapping and Gene Expression Analysis through Microarray
Rice (Oryza sativa L.) is a model organism for the functional genomics of monocotyledonous plants since the genome size is considerably smaller than those of other monocotyledonous plants. Although highly accurate genome sequences of indica and japonica rice are available, additional resources such as full-length complementary DNA (FL-cDNA) sequences are also indispensable for comprehensive ana...
متن کاملFull-length cDNA, Expression Pattern and Association Analysis of the Porcine FHL3 Gene
Four-and-a-half LIM-only protein 3 (FHL3) is a member of the LIM protein superfamily and can participate in mediating protein-protein interaction by binding one another through their LIM domains. In this study, the 5'and 3'cDNA ends were characterized by RACE (Rapid Amplification of the cDNA Ends) methodology in combination with in silico cloning based on the partial cDNA sequence obtained. Bio...
متن کاملGene expression profiling analysis of hepatocellular carcinoma
BACKGROUND Primary hepatocellular carcinoma (HCC) is one of the most common malignancies in the world. However, the molecular pathogenesis of HCC is not well-understood, and the prognosis for patients with HCC remains very poor. METHODS To disclose detailed genetic mechanisms in hepatocellular carcinoma (HCC) with a view toward development of novel therapeutic targets, we analyzed expression ...
متن کاملClassification of Hepatocellular Carcinoma by Gene Expression Profiling Analysis
Previous papers reported the effectiveness in classification of cancers with their gene expression portraits [1, 2, 3]. Golub et al. [3] selected 50 genes of high predictive value to differentiate 2 types of leukemia by ‘weighted voting algorithm’. However it remains to be known whether we can apply this type of specific algorithms to classify different types of cancer. We, therefore, attempted...
متن کاملGeneration of full-length cDNA library from single human prostate cancer cells.
Several existing methods to construct cDNA libraries require several thousand cells and involve the lengthy procedure of reverse transcription (RT), restriction, adaptor ligation and vector cloning (5,6), which usually fail to maintain the completeness of a cDNA library, resulting in a loss of rare cDNAs. However, gene expression analysis of specific cell populations within a heterogeneous tiss...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biomedical Science
سال: 2006
ISSN: 1021-7770,1423-0127
DOI: 10.1007/s11373-005-9062-6